Research Assistant (Biological Sciences)

Interested applicants are invited to apply directly at the NUS Career Portal. Please note your application will only be processed if you apply via NUS Career Portal.

NUS Career Portal link: https://careers.nus.edu.sg/job/Research-Assistant-(Biological-Sciences)/34899-en_GB/?st=C0A4BEE63C00D183B283B399D0D3940AF6AC43C2

We regret that only shortlisted candidates will be notified.

We are seeking a motivated Research Assistant to support a research project investigating sexual development and reproductive biology in Red Snapper. The project aims to produce recombinant fish gonadotropins using Pichia pastoris, develop antibodies and analytical methods to measure gonadotropins, and apply these tools to study reproductive development and support the completion of the Red Snapper reproductive life cycle.

The appointee will conduct laboratory research involving recombinant protein expression, purification and characterisation, antibody production, and ELISA development and analysis. The role will also involve analysing gonadotropin levels in Red Snapper samples, reviewing relevant literature, interpreting results, and contributing to scientific reports and presentations.

Key Responsibilities

• Produce recombinant fish gonadotropins using Pichia pastoris, including expression optimisation and culture work
• Purify and characterise recombinant proteins using appropriate biochemical and analytical methods
• Generate antibodies against recombinant gonadotropins and assess their suitability for research applications
• Develop, optimise and perform ELISA assays to detect and quantify gonadotropins
• Analyse gonadotropin levels in Red Snapper samples and interpret results in relation to sexual development and reproductive status
• Maintain accurate records of experiments, sample information, and research data
• Conduct literature reviews on fish gonadotropins, reproductive development, and aquaculture
• Contribute to data analysis, scientific reporting, presentations, and preparation of research outputs

Qualifications

Degree in a relevant discipline, such as Molecular Biology, Biochemistry, Biotechnology, Aquaculture, or a related field

Experience

• Formal training in molecular biology, protein biochemistry, or related laboratory techniques
• Recombinant protein expression, preferably using Pichia pastoris
• Protein purification and characterisation
• ELISA development or immunoassays
• Experience with antibody production or antibody validation would be an advantage
• Familiarity with fish biology, reproductive physiology, or aquaculture would be an advantage
• Experience handling fish samples or analysing biological samples would be an advantage
• Ability to work independently, maintain accurate experimental records, and follow laboratory protocols rigorously
• Strong organisational skills, with the ability to manage experiments and meet project timelines
• Good written and verbal communication skills, including the ability to contribute to literature reviews, reports, and presentations
• Willingness to support routine laboratory operations, including equipment care, inventory management, and housekeeping